Journal: Journal of Bacteriology

Article Title: Natural Strain Variation Reveals Diverse Biofilm Regulation in Squid-Colonizing Vibrio fischeri

doi: 10.1128/JB.00033-19

Figure Lengend Snippet: DNA oligonucleotides for PCR amplification and sequencing

Article Snippet: Full inserts from all cloned constructs were verified by Sanger DNA sequencing through ACGT, Inc., via the Northwestern University Feinberg School of Medicine NUSeq Core Facility or the University of Wisconsin—Madison Biotechnology Center.

Techniques: Amplification, Sequencing

Journal: Frontiers in Immunology

Article Title: Role of Dsg1- and Dsg3-Mediated Signaling in Pemphigus Autoantibody-Induced Loss of Keratinocyte Cohesion

doi: 10.3389/fimmu.2019.01128

Figure Lengend Snippet: Genetic deletion of Dsg3 and Dsg2 using CRISPR/Cas9 in HaCaT keratinocytes. (A) Schematic of sequencing results after inducing a DSB with NHEJ repair in Exon 5 of either Dsg3 or Dsg2 using CRISPR/Cas9. (B) Cell line characterization by immunostaining of desmosomal proteins ( n = 3). (C) Cell line characterization by immunoblot with representative images on the left and densitometric quantification on the right ( n = 3, one-way ANOVA, * p ≤ 0.05 vs. WT). (D) Dispase assay of WT and Dsg-deficient cell lines under baseline treatment. ( n = 7, one-way ANOVA, * p ≤ 0.05 vs. WT) (E) Cell lines incubated with IgG fractions for 24 h and subjected to Dispase assays ( n = 5, two-way ANOVA, # p ≤ 0.05 vs. respective c-IgG, * p ≤ 0.05 vs. WT).

Article Snippet: Afterwards, genomic DNA was extracted using a standard Phenol-Chloroform DNA extraction protocol and send for Sanger sequencing with an area of 500 base pairs flanking both ends of the target site (Eurofins, Ebersberg, Germany).

Techniques: CRISPR, Sequencing, Immunostaining, Western Blot, Incubation